New Study Sheds Light on the Impact of Diabetes on Root Canal Dentin
Introduction
A recent groundbreaking study by Mohammad Ali Saghiri and colleagues has utilized high-resolution transmission electron microscopy (HRTEM) and inductively coupled plasma mass spectrometry (ICP-MS) to assess the impact of diabetes mellitus (DM) on the nanostructure of root canal dentin.Approach
The researchers examined twenty extracted human premolars procured from both diabetic and non-diabetic patients (n=10 in each group). The teeth were decoronated and split horizontally into forty 2-mm-thick dentin discs, each assigned to a specific test. The ICP-MS method was employed to ascertain the varying elemental levels of Cu, Li, Zn, Se, Sr, Mn, and Mg in diabetic and non-diabetic specimens. HRTEM was utilized for analyzing the form and quantity of the apatite crystals in diabetic and non-diabetic dentin at the nanostructural level. The data was then subjected to statistical analysis using Kolmogorov-Smirnov and student’s t-tests (p<0.05).Findings
The ICP-MS analysis unveiled significant variances in trace element concentrations between diabetic and non-diabetic specimens (p<0.05). Strikingly, diabetic specimens displayed lower levels of Mg, Zn, Sr, Li, Mn, and Se (p<0.05), and higher levels of Cu (p<0.05). The HRTEM analysis further revealed that diabetic dentin had a less compact structure with smaller crystallites and significantly more crystals in the 2500 nm2 area (p<0.05).Free for members
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