CRISPR: Reshaping Future - An Overview

“Any sufficiently advanced technology is indistinguishable from magic.”
~Arthur C. Clarke

It is an axiomatic fact to say that technology has surpassed mankind in every aspect. What if I told you that there is something at the skyline that would prove to be the most transformative contrivance so far? Hereby, introducing CRISPR!

CRISPR: What is it?

CRISPR is an abbreviation that expands to ‘Clustered Regularly Interspaced Short Palindromic Repeats’. As much as the name in itself is a bit complex, it simply means a programmable protein that not only edits, or eliminates, but has the potential to switch on/off genes. Yes, you got that right; it’s a technology that is designed to orchestrate the genetic code, including human DNA.

CRISPR works on the Cas9 technology used by bacteria and archaea which is an RNA-guided genome editing tool to obliterate the viral DNA, delivering immunity to the host against viral nucleic acids. Cas9 proteins are popularly known as “DNA locator systems” since they can be programmed by scientists to target whatever piece of DNA they decide to send it to. Once it is there, they could be licensed with the power to cut out a particular gene, re-activate/inactivate a gene, or replace a gene with something different. Hence, the CRISPR-Cas9 system finds its role in medicine as a powerful therapeutic tool in treating diseases that occur as a result of genomic mutations.

CRISPR’s Miraculous Contribution to Dentistry

Despite the controversial nature of CRISPR as to how, and even if it is to be used at all, paired with the fact that it’s still in its infancy, CRISPR-Cas9 technology finds several potential applications in the field of dentistry, all of which are aimed at bringing a revolution in the dental era. Nevertheless, the practical execution of these uses could take years to develop and implement CRISPR treatments into a dental clinical setup.

However, there have been pieces of evidence that point to the possibility of treatments for some of the most debilitating dental conditions through this technology. Various uses of CRISPR in dentistry found so far are as follows:

• Possible new treatment for oral cancer – Oral Squamous Cell Carcinoma (OSCC) is the most common type of oral cancer that remains a disease with a low survival rate and scarce exceptional treatment options over decades. CRISPR-Cas9 is an emerging technology that has great efficacy in identifying genes responsible for oral cancer pathobiology and assisting in the treatment of the same by gene knockout technique as suggested by Huang et al in 2017 by studying the role of p75NTR gene on human tongue carcinoma. According to this study, several properties of SCC-9 cells are suppressed by the deletion of the p75NTR gene.

• Inhibition of plaque formation − As we know well, Streptococcus mutans is the primary bacteria in the oral cavity that creates a biofilm by utilizing sucrose to synthesize an extracellular polysaccharide (EPS) in which the bacterial colonies live and multiply in leading to dental plaque formation. Surprisingly, in a study by Gong T et al, self-targeting CRISPR arrays were formulated and cloned onto plasmids which were later transformed to UA159, acquiring desired mutants that significantly reduced EPS formation and thus, increased breakdown of the biofilm.

While these are some exciting prospects, the studies are still in their preliminary stages and require much more research to bring a technology like this to fruition. But the possibility exists!