Guided bone regeneration (GBR) is a commonly used procedure for regenerating bone defects, often employed in dental surgeries. This study aimed to investigate the adherence of Candida albicans on six commercially available polytetrafluoroethylene (PTFE) membranes used in GBR procedures and to understand the clinical implications of such adherence.
Materials and Methods
The study evaluated six PTFE membranes: two with textured surfaces and four with non-textured surfaces. C. albicans (ATCC 24433) was cultured for 24 hours, and its cell surface hydrophobicity was assessed using a modified method. The adhesion of C. albicans to membrane discs was analyzed using scanning electron microscopy (SEM) and real-time polymerase chain reaction (PCR).Results
- albicans exhibited significant hydrophobicity at 77.25%. SEM analysis revealed patchy, scattered, and small clustered patterns of C. albicans adherence across all membranes except for one non-textured membrane with a rough surface where a thick biofilm formed. Real-time PCR quantification showed significantly higher adhesion levels on PTFE membranes compared to control membranes (p ≤. 001). Textured surfaces had the highest count of adhered cells at 2680 × 10^4 cells/ml compared to non-textured surfaces at 707 × 10^4 cells/ml (p ≤. 001). One particular rough-surfaced non-textured membrane exhibited the highest count at 3010 × 10^4 cells/ml (p ≤. 05).
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