A recent study has revealed that sanitization of toothbrushes with mouthwash can effectively reduce the number of live microorganisms adhered to the filaments of toothbrushes.
Toothbrushes are colonized by microorganisms, implying a risk of infection. By reducing this microbial colonization, the risk of disease transmission can be reduced significantly. Therefore, the purpose of this study was to evaluate the antiseptic activity of a mouthwash containing 0.05% chlorhexidine and 0.05% cetylpyridinium chloride on toothbrushes.
A total of twelve toothbrushes used three times/day for 14 days by orally and systemically healthy people were randomly split into two groups, and their heads were immersed for 2 h in PBS (control) or Perio·Aid Active Control (treatment). The microorganisms were recovered, and their number was calculated by culture, quantitative PCR, and viability PCR. Statistical differences were first assessed with a two-way mixed ANOVA and subsequently with Student's t-test.
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